• DNA Extraction Kits

Animal Tissue DNA Extraction Kit

The InoMag Animal Tissue DNA Isolation Kit provides all critical reagents necessary for the isolation of high-molecular-weight genomic DNA from a broad range of animal tissues for multiple downstream applications such as PCRs, qPCRs and sequencing Suitable for processing all kinds of animal tissue types such as fibrous or elastic tissues such as lung, muscle, and also from soft tissues such as liver, kidney, prostate, and brain. The InoMag animal tissue DNA Extraction uses highly functionalized magnetic beads that enables rapid capture of very intact DNA from tissue isolates.

The high affinity binding of DNA to the magnetic beads with large surface area ensures maximum yield and purity of isolated DNA. This kit can be used for genomic DNA extraction from tissues of both animal and mammals and cell lines. InoMag animal tissue isolation kit is capable for isolation of high-quality genomic DNA in better yields compared to other commercially available kits from a tissue volumes of 10-50 mg.

Special Features

  • Highly efficient yields up to 50 μg, depends on type of sample.
  • Pure and intact genomic DNAready-to-use in all Molecular Biology applications.
  • Easy and fast procedure aspurification of total DNA from a variety of tissues takes lesser time with minimal handling steps.
  • Standardized and easy to follow protocol
  • No organic solvent extraction is required
  • Inhibitor-free DNA which can be readily used for further downstream applications

Blood DNA Extraction Kit

InnoMag Blood DNA Extraction kit is meant for rapid and easy purification of nucleic acids from blood samples. Our highly functionalised Magnetic beads ensures high quality isolation of DNA from blood with a volume ranges of 0.2 – 1.0 ml. Our kit helps in overcoming the time-consuming and limiting conventional methods with our optimized protocol for a quick and reproducible extraction with better yields. DNA extracted is pure and intact and suitable for all downstream applications such as PCR, Real time PCR, Restriction digestion, Sanger sequencing, NGS and Southern blotting.

Special Features

  • The kit is compatible with any type of vacutainer (Citrate/EDTA/fluoride/heparin tubes)
  • No organic solven extraction like phenol required
  • Minimal centrifugation steps
  • Gives good results even with blood clots, aged blood samples and blood spots

Special Features

  • Rapid purification of high-quality, ready-to-use DNA
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors
  • Magnetic bead-based purification
  • Automation friendly

cfDNA Extraction Kit

The InnoMag Cell-Free DNA Isolation Kit is designed for enrichment of circulating cell-free DNA (cfDNA) from  biological samples such as serum and plasma. cfDNA recovery is challenging due to its very limited amount present in cell-free samples. The kit is based on magnetic-bead technology, enabling reproducible recovery of high-quality DNA that is suitable for a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing.

Magnetic beads offer many benefits compared to other technologies for isolating cfDNA. Beads bind the DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields. The InnoMag Cell-Free DNA Isolation Kit addresses this with its magnetic bead-based purification format which allows to easily process a wide range of sample input volumes, from 100 to 1000 µL. InnoMag cfDNA kit can be adapted for automation with many liquid handling robots equipped with magnetic seperators. Alternatively, samples can be processed manually with the use of a  easy to use magnetic stand.

Special Features

  • Flexible design that permits both manual and automated isolation of cfDNA
  • Phenol-free extraction
  • Wide sample volume inputs range from 100  to 1000µL of plasma/serum or urine
  • Contains highly functionalized magnetic beads for consistent isolation of cfDNA
  • Elution volumes range from 20 to 50 µL

FFPE Tissue DNA Extraction Kit

The InnoMag DNA FFPE Tissue Kit is specially designed for purifying DNA from formalin-fixed, paraffin-embedded tissue sections. The kit uses special lysis conditions to release DNA from tissue sections and to overcome inhibitory effects caused by formalin crosslinking of nucleic acids. Isolated DNA is high in concentration, formaldehyde crosslinks are reversed efficiently, and the DNA quality of the sample is well preserved during the complete process of deparaffinization, de-crosslinking, and purification.

Thus, isolated DNA shows good performance in typical downstream applications such as PCR, qPCR and sequencing.  The kit uses functionalized magnetic beads  for purification of high-quality DNAfrom scalable volumes of samples..

Special Features

  • Rapid purification of high-quality, ready-to-use DNA
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors
  • Magnetic bead-based purification
  • Automation friendly

InnoMAg Plant DNA Isolation Kit

The InnoMAg plant DNA isolation kit is intended for manual or automated extraction of Genomic DNA from Plant Tissues like leaf, seeds, stem, roots and other parts of Plant Tissues samples. This kit is adaptable for use on major liquid handling workstations for high throughput processing. The whole operation process is safe, convenient and no need for organic reagent. The extracted nucleic acids are highly purified with optimum quantity, can be widely used for PCR Amplification, Sanger and Next Generation sequencing, Fragment analysis, Genotyping and SNP detection, Restriction enzyme clean-up for wide branch of diagnostics, genomics research, disease detection, food safety and forensic identification, etc.

Plant Nucleic acid extraction principle is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate pH conditions. Plant tissues are crushed with Lysis Buffer, followed by binding of nucleic acid to paramagnetic beads with Binding Buffer, the bound nucleic acids are cleaned up with wash buffers twice to remove contaminants. Finally, highly purified DNA is eluted with low salt Elution Buffer. The purified DNA can directly be used for downstream applications such as PCR, qPCR and Sequencing.

Special Features

  • Suitable for high quality and yields of DNA from many plant and crop species
  • Suitable for many tissue types such as leaves, seeds, stem, roots or any plant parts
  • Can be used for both manual and automated extraction

Plant DNA Extraction Kit

The InnoMag Plant DNA extraction Kit allows rapid and reliable isolation of high-quality genomic DNA from multiple tissues of vast plant species. The lysis and binding buffers are specifically designed to eliminate interfering polysaccharides and polyphenols. Samples of 50 mg wet tissue (or 15 mg dry tissue) can be processed in parallel in less than one hour. The system combines CTAB-based lysis, which eliminates the need for organic solvents, with the convenience of functionalized magnetic beads that promotes specific binding of nucleic acids and eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.

This kit is designed for both manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. Purified DNA is suitable for all downstream applications like PCR, restriction digestion, next-generation sequencing, and hybridization applications. There are no organic extractions thereby reducing consumables and decreasing hands-on time to allow multiple samples to be processed together.

Special Features

  • Kit is capable of offering very high yields of very intact and pure DNA than many of the commercial kits from multiple plant species
  • Suitable for both manual and automated DNA extraction
  • Works with multiple tissues like leaves, seeds, stem bits, floral tissues etc.

Saliva DNA Extraction Kit

Saliva represents an excellent non-invasive alternative to blood collection.  Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose many diseases and germline genetic disorders, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose multiple types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.

The InnoMag Saliva DNA Isolation Kit offersboth manual and automatable magnetic bead-based genomic DNA purification from fresh and stabilized saliva. The resulting purified DNA is suitable for multip[le downstream  applications such as PCR, qPCR, next-generation sequencing, and microarray analysis. The InnoMag Saliva DNA Isolation Kit isolates gDNA from 200 to 1000 µL–of freshly collected saliva or stabilized saliva collected using a commercially available saliva collection tubes. The InnoMag Saliva DNA Isolation Kit combines multiple laborious sample processing steps together into a single step to minimize the time and effort typically required by other methods, without compromising yield and purity. The InnoMag Saliva gDNA Isolation Kit maximizes gDNA yields by treating samples with a powerful lysis/binding chemistry to simultaneously lyse cells, denature proteins, and capture gDNA on highly functionalised magnetic binding beads

Special Features

  • Can be adopted for both manual and automated extractions
  • Process up to 96 samples in 45 minutes
  • Isolate gDNA from 200 to 1000 µLof saliva using simple protocols in SBS deepwell plates
  • Reproducible extraction of high molecular weight genomic DNA purified.
  • Safe and convenient as the kit avoids phenol/chloroform Extraction.
  • Efficient for extractions upto 5-20 μg (depending on the sample material amount and type).
  • Pure genomic DNA: ready-to-use for all downstream applications